Khao Soi Dao wildlife sanctuary is an important hotspot for orchid diversity. This study aimed to document selected ecological aspects, assess reproductive success and compare asymbiotic seed germination among Phalaenopsis cornu-cervi (Breda) Blume & Rchb. f., P. deliciosa Rchb. f. and P. difformis (Wall. ex Lindl.) Kocyan & Schuit. within the sanctuary. In the 10 × 10 m² samplingplots, the occurrence frequency and blooming percentage of P. cornu-cervi and P. deliciosa were recorded. The results showed that P. cornu-cervi had a higher occurrence frequency and a greater proportion of blooming individuals than P. deliciosa. The assessment of reproductive success indicated that the fruit-set percentages of P. cornu-cervi, P. deliciosa and P. difformis were 1.64, 14.09 and 21.23%, respectively. In the asymbiotic seed germination experiment, Vacin and Went (VW) medium yielded the highest germination percentage for P. cornu-cervi (81.7%), followed by MS (72.8) and ½ MS (47.2%). Similarly, for P. difformis, VW medium induced significantly higher germination (14.8) than MS (1.2) and ½ MS (5.3%). In contrast, seeds of P. deliciosa failed to germinate on any culture medium tested. These findings provide basic information for the reintroduction and recovery of natural populations, as well as guidelines for propagation via seed of these species.