Thai Journal of Botany
Article's details
Title: Morphological and molecular characterization of Fusarium spp., the fungi associated with mulberry root rot disease in north-eastern Thailand
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Pages: 25 - 39
Year: 2553
Year No.: 2
Volume: 1   Show All Articles
Abstract:         This study was conducted to isolate and identify Fusarium spp. the associated causal agents of mulberry root rot disease. One hundred and  fty-seven isolates of Fusarium spp. were obtained from 5 mulberry plantations during 2007 to 2008. All species were identied morphologically with particular emphasis on macroconidia, microconidia, the size and types of conidiophores, and the presence or absence of chlamydospores. The species associated with mulberry roots and surrounding rhizosphere were identied to 11 species including 102 isolates of Fusarium solani, 26 of F. moniliforme, 16 of F. oxysporum, 3 each of F. phaseoli and F. dlamini, 2 of F. culmorum, 1 each of F. dimerum, F. graminearum, F. beomiforme, F. scirpi and F. anthophilum. Genetic variation of 30 isolates of Fusarium spp. was studied using random amplied polymorphic DNA (RAPD) markers. Four primers A02, A03, A07 and A09 produced  fingerprint profles, which clearly distinguished between the different species of Fusarium spp. The high level of genetic variability among species of Fusarium allowed fingerprinting of most of the isolates by examining patterns produced by several primers. The results suggest that RAPD-PCR is a useful method for analyzing genetic variation within and between Fusarium spp.
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